secondary antibody dilution western blot

15779-1-AP. WB analysis using 11587-1-AP. Supplied as 2 mL purified secondary antibody (0.8 mg/mL). We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. The main consideration when choosing a lysis buffer is whether the chosen antibody will recognize denatured samples. For signal development, follow the kit manufacturers recommendations. Untreted and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. Invitrogen Anti-Mouse IgG (H+L) Secondary Antibody, Catalog # 31430. The enzyme-linked immunosorbent assay (ELISA) (/ l a z /, / i l a z /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. Performing quantitative western blots or long duration is needed: For routine western blots or new systems not yet optimized: Detecting high abundant protein targets and sample is abundant: Recommended primary antibody dilutions, from a 1mg/mL (0.21 g/mL) (0.021 g/mL) (0.21 g/mL) (0.210 g/mL) The main consideration when choosing a lysis buffer is whether the chosen antibody will recognize denatured samples. WB analysis of rat skin using 22734-1-AP The membrane can then be processed with primary antibodies specific for target proteins of interest. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight 488 (IgG; H+L) used at a 1/250 dilution for 1h. Return to the antibody guide. Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight 488 (IgG; H+L) used at a 1/250 dilution for 1h. POLR2L Polyclonal antibody. When this is not the case, it will be noted on the antibody datasheet, and buffers without detergent or with relatively mild non-ionic detergents (NP-40, Triton X-100) should be used. human igg and human serum. human igg and human serum. Return to the antibody guide. Visualization with the Opti-4CN chromogenic substrate kit (BioRad). Visualization with the Opti-4CN chromogenic substrate kit (BioRad). Prepare the secondary antibody dilution with 0.05% Tween 20 detergent. An overview of western blot analysis covering protein separation Another common technique is to add a 1:10 dilution of the blocking solution to the wash buffer. Supplied as 2 mL purified secondary antibody (0.8 mg/mL). The Western blot cocktail is supplied at a concentration of 1.5 mg/mL. mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. 14820-1-AP. The membrane can then be processed with primary antibodies specific for target proteins of interest. High background from an excess of secondary antibody: Optimize the secondary antibody dilution depending on the dye being used, following the vendor-recommended dilution and adapting accordingly. WB analysis of rat kidney using 19987-1-AP Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The sample is counter-stained with hematoxylin. Print a copy of our western blot protocol The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight 488 (IgG; H+L) used at a 1/250 dilution for 1h. The enzyme-linked immunosorbent assay (ELISA) (/ l a z /, / i l a z /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. Dilution; Western Blot (WB) WB : 1:1000-1:4000: Immunoprecipitation (IP) View protocol for Electrophoresis and Protein Transfer. The sample is counter-stained with hematoxylin. The Western blot cocktail is supplied at a concentration of 1.5 mg/mL. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Invitrogen Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Catalog # A32723. Untreated and PNGase F-treated lysates of THP-1 cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Akt antibody confirms silencing of Akt expression, while the p42 MAP kinase (Erk2) antibody is used to control for loading and Untreated and PNGase F-treated lysates of THP-1 cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Return to the antibody guide. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Use the antibody dilution calculator to assist with calculating antibody volumes based on incubation volume. Invitrogen Anti-Mouse IgG (H+L) Secondary Antibody, Catalog # 31430. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: HeLa, HEK293T; Related Products. Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments. GPR37/Pael-R Polyclonal antibody. Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified ab32063 at a working dilution of 1 in 200. Increase the number or duration of wash steps. A549 cells were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Applications: Western Blot, Primary Antibody Dilution: 1:500; Cell Tissue Type: RPE-1 cell lysates (6cm dish) Related Products. The cells were then incubated with the antibody (ab8898, 0.1g/ml) overnight at +4C. Applications: Western Blot, Primary Antibody Dilution: 1:5000; Cell Tissue Type: Rat Dorsal Root Ganglia; GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1 Prepare dilutions of the conjugated secondary antibody in appropriate volume of wash buffer or alternatively in blocking buffer. The enzyme-linked immunosorbent assay (ELISA) (/ l a z /, / i l a z /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. Adjust concentration of milk up or down to obtain desired signal strength and low background. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. 16093-1-AP. Secondary antibodies optimized for WB. Applications: Western Blot, Primary Antibody Dilution: 1:500; Cell Tissue Type: RPE-1 cell lysates (6cm dish) Related Products. 16093-1-AP. Secondary Antibodies Antibody Labeling Kits New; ELISA Kits A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. A wide variety of labeled secondary antibodies can be used for western blot detection. GPR37/Pael-R Polyclonal antibody. PNGase F Untreted and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. pig skin tissue were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. 14820-1-AP. Akt antibody confirms silencing of Akt expression, while the p42 MAP kinase (Erk2) antibody is used to control for loading and Incubate the membrane with the recommended dilution of conjugated secondary antibody in blocking buffer at room temperature for 1 h. Wash the membrane in three washes of TBST, 5 min each. Next, secondary antibodies bound to enzymes are applied and finally a substrate that reacts with the secondary antibody-bound enzyme is added for detection of the antibody/protein complex. Store the antibody cocktail at 4C and the control sample at -80C. GPR37/Pael-R Polyclonal antibody. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. WB analysis using 10427-2-AP. The membrane can then be processed with primary antibodies specific for target proteins of interest. mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Prepare dilutions of the conjugated secondary antibody in appropriate volume of wash buffer or alternatively in blocking buffer. Popular 30 mins at RT. Supplied as 1 mg purified secondary antibody (2 mg/mL). Print a copy of our western blot protocol Please read the following Western blot cell lysate protocol in its entirety before beginning. High background from an excess of secondary antibody: Optimize the secondary antibody dilution depending on the dye being used, following the vendor-recommended dilution and adapting accordingly. and 2% nonfat dry milk for primary and secondary antibody dilution. View our western blot protocol video below. WB analysis of rat kidney using 19987-1-AP Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. WB analysis of mouse lung using 14695-1-AP The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to WB analysis using 10427-2-AP. Invitrogen Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Catalog # A32723. Applications: Western Blot; Primary Antibody Dilution: 1:1000; Cell Tissue Type: 293T; Beta Actin Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66009-1-Ig) Akt antibody confirms silencing of Akt expression, while the p42 MAP kinase (Erk2) antibody is used to control for loading and Increase the number or duration of wash steps. Performing quantitative western blots or long duration is needed: For routine western blots or new systems not yet optimized: Detecting high abundant protein targets and sample is abundant: Recommended primary antibody dilutions, from a 1mg/mL (0.21 g/mL) (0.021 g/mL) (0.21 g/mL) (0.210 g/mL) Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human platelets A wide variety of labeled secondary antibodies can be used for western blot detection. Invitrogen Anti-Mouse IgG (H+L) Secondary Antibody, Catalog # 31430. and 2% nonfat dry milk for primary and secondary antibody dilution. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. WB analysis of mouse lung using 14695-1-AP Supplied as 2 mL purified secondary antibody (0.8 mg/mL). Incubate the membrane with the recommended dilution of conjugated secondary antibody in blocking buffer at room temperature for 1 h. Wash the membrane in three washes of TBST, 5 min each. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human breast cancer cells; KEAP1 Antibody Western Blot, validation (1:1000 dilution) in Human breast cancer cells (Cat no:10503-2 A549 cells were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. 15779-1-AP. Protein location and lysis buffer choice Popular 30 mins at RT. Untreated and PNGase F-treated lysates of THP-1 cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Protein location and lysis buffer choice IP=immunoprecipitation, WB=western blot. Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. pig skin tissue were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. POLR2L Polyclonal antibody. Secondary Antibodies Antibody Labeling Kits New; ELISA Kits A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. Popular 30 mins at RT. PNGase F POLR2D Polyclonal antibody. human igg and human serum. Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human platelets High background from an excess of secondary antibody: Optimize the secondary antibody dilution depending on the dye being used, following the vendor-recommended dilution and adapting accordingly. Prepare dilutions of the conjugated secondary antibody in appropriate volume of wash buffer or alternatively in blocking buffer. WB analysis of HeLa using 60291-1-Ig Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. WB analysis of mouse lung using 11257-1-AP Adjust concentration of milk up or down to obtain desired signal strength and low background. Adjust concentration of milk up or down to obtain desired signal strength and low background. View our western blot protocol video below. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human breast cancer cells; KEAP1 Antibody Western Blot, validation (1:1000 dilution) in Human breast cancer cells (Cat no:10503-2 Supplied as 1 mg purified secondary antibody (2 mg/mL). Next, secondary antibodies bound to enzymes are applied and finally a substrate that reacts with the secondary antibody-bound enzyme is added for detection of the antibody/protein complex. Increase the number or duration of wash steps. An overview of western blot analysis covering protein separation Another common technique is to add a 1:10 dilution of the blocking solution to the wash buffer. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human breast cancer cells; KEAP1 Antibody Western Blot, validation (1:1000 dilution) in Human breast cancer cells (Cat no:10503-2 Applications: Western Blot; Primary Antibody Dilution: 1:1000; Cell Tissue Type: 293T; Beta Actin Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66009-1-Ig) Incubate the membrane with the recommended dilution of conjugated secondary antibody in blocking buffer at room temperature for 1 h. Wash the membrane in three washes of TBST, 5 min each. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. 16093-1-AP. The Western blot cocktail is supplied at a concentration of 1.5 mg/mL. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. When this is not the case, it will be noted on the antibody datasheet, and buffers without detergent or with relatively mild non-ionic detergents (NP-40, Triton X-100) should be used. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D) Related Products. Dilution; Western Blot (WB) WB : 1:1000-1:4000: Immunoprecipitation (IP) Prepare the secondary antibody dilution with 0.05% Tween 20 detergent. Untreted and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. The sample is counter-stained with hematoxylin. View our western blot protocol video below. WB analysis of mouse lung using 14695-1-AP Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments. A wide variety of labeled secondary antibodies can be used for western blot detection. 14820-1-AP. Applications: Western Blot, Primary Antibody Dilution: 1/1000; Cell Tissue Type: ST cells (porcine) YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP) Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and ELISA (ELISA) applications. Use the antibody dilution calculator to assist with calculating antibody volumes based on incubation volume. POLR2L Polyclonal antibody. Please read the following Western blot cell lysate protocol in its entirety before beginning. WB analysis of HeLa using 60291-1-Ig Visualization with the Opti-4CN chromogenic substrate kit (BioRad). The cells were then incubated with the antibody (ab8898, 0.1g/ml) overnight at +4C. IP=immunoprecipitation, WB=western blot. POLR2D Polyclonal antibody. We ordered this actin antibody to detect actin as internal control in the cell lysates during the western blot, and the antibody is very sensitive. WB analysis of rat skin using 22734-1-AP WB analysis of mouse lung using 11257-1-AP We ordered this actin antibody to detect actin as internal control in the cell lysates during the western blot, and the antibody is very sensitive. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. An overview of western blot analysis covering protein separation Another common technique is to add a 1:10 dilution of the blocking solution to the wash buffer. WB analysis of rat kidney using 19987-1-AP Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. A549 cells were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to When this is not the case, it will be noted on the antibody datasheet, and buffers without detergent or with relatively mild non-ionic detergents (NP-40, Triton X-100) should be used. pig skin tissue were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. Performing quantitative western blots or long duration is needed: For routine western blots or new systems not yet optimized: Detecting high abundant protein targets and sample is abundant: Recommended primary antibody dilutions, from a 1mg/mL (0.21 g/mL) (0.021 g/mL) (0.21 g/mL) (0.210 g/mL) Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. WB analysis of rat skin using 22734-1-AP Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000; Cell Tissue Type: Human platelets Applications: Western Blot, Primary Antibody Dilution: 1:500; Cell Tissue Type: RPE-1 cell lysates (6cm dish) Related Products. POLR2D Polyclonal antibody. WB analysis using 10427-2-AP. We ordered this actin antibody to detect actin as internal control in the cell lysates during the western blot, and the antibody is very sensitive. WB analysis of mouse lung using 11257-1-AP 15779-1-AP. Applications: Western Blot, Primary Antibody Dilution: 1/1000; Cell Tissue Type: ST cells (porcine) YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP) Applications: Western Blot, Primary Antibody Dilution: 1/1000; Cell Tissue Type: ST cells (porcine) YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP) Applications: Western Blot, Primary Antibody Dilution: 1:5000; Cell Tissue Type: Rat Dorsal Root Ganglia; GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1 For signal development, follow the kit manufacturers recommendations. Supplied as 1 mg purified secondary antibody (2 mg/mL). View protocol for Electrophoresis and Protein Transfer. and 2% nonfat dry milk for primary and secondary antibody dilution. IP=immunoprecipitation, WB=western blot. Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. Next, secondary antibodies bound to enzymes are applied and finally a substrate that reacts with the secondary antibody-bound enzyme is added for detection of the antibody/protein complex. Secondary antibodies optimized for WB. The main consideration when choosing a lysis buffer is whether the chosen antibody will recognize denatured samples. Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Dilution; Western Blot (WB) WB : 1:1000-1:4000: Immunoprecipitation (IP) Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D) Related Products. WB analysis of HeLa using 60291-1-Ig Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: HeLa, HEK293T; Related Products. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: HeLa, HEK293T; Related Products. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. Secondary antibodies optimized for WB. Protein location and lysis buffer choice We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. For signal development, follow the kit manufacturers recommendations. Please read the following Western blot cell lysate protocol in its entirety before beginning. Applications: Western Blot; Primary Antibody Dilution: 1:1000; Cell Tissue Type: 293T; Beta Actin Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66009-1-Ig) Secondary Antibodies Antibody Labeling Kits New; ELISA Kits A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The cells were then incubated with the antibody (ab8898, 0.1g/ml) overnight at +4C. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and ELISA (ELISA) applications. Print a copy of our western blot protocol Prepare the secondary antibody dilution with 0.05% Tween 20 detergent. Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. We recommend using a high pH CAPS / PVDF transfer protocol when using this antibody for Western blot. Store the antibody cocktail at 4C and the control sample at -80C. Use the antibody dilution calculator to assist with calculating antibody volumes based on incubation volume. Store the antibody cocktail at 4C and the control sample at -80C. Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Applications: Western Blot, Primary Antibody Dilution: 1:1000; Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D) Related Products. View protocol for Electrophoresis and Protein Transfer. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and ELISA (ELISA) applications. 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